Journal article

Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5

EM Menkhorst, ML Van Sinderen, K Rainczuk, C Cuman, A Winship, E Dimitriadis

SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2017

Abstract

During the establishment of pregnancy, extravillous trophoblast (EVT) must invade into the uterine decidua to facilitate decidual artery remodelling to create the placental blood supply. The local decidual environment is thought to regulate trophoblast invasion, however these interactions are poorly defined in humans. Recent evidence in women suggests impaired decidualization is associated with miscarriage and preeclampsia. Primary human endometrial stromal cells (HESC) and first trimester extravillous trophoblast (EVTs) were used to assess the effect of EVT-secreted factors on HESC decidualization, adhesion, proliferation and migration. We determined the role of profilin (PFN)1, an EVT-secr..

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Grants

Awarded by NHMRC (Australia)


Funding Acknowledgements

The authors would like to acknowledge the invaluable contribution of our research nurse Sister Judi Hocking for the consenting of patients and collection of tissues. We would also like to thank Dr Jemma Evans, Dr Tara Krishnan, Natalie Lane, Priscilla Li, Dr Sarah Paule, Dr Stefan Sonderegger, Thalini Gamage and Joanne Yap for their assistance with tissue isolation. This work was supported by NHMRC (Australia) Project/Program Grant (GNT1027100) to ED and EM and by the Victorian Government's Operational Infrastructure Support Program. ED was supported by NHMRC (Australia) senior research fellowship (#550905). EM was supported by NHMRC (Australia) early career fellowship (#611827). CC and AW were supported by an Australian Postgraduate Award (Australia). The funding bodies had no role in the study design, collection, analysis, or interpretation of data or in the writing of the manuscript.