Interleukin-11 up-regulates endoplasmic reticulum stress induced target, PDIA4 in human first trimester placenta and in vivo in mice
AL Winship, K Sorby, J Correia, A Rainczuk, J Yap, E Dimitriadis
PLACENTA | W B SAUNDERS CO LTD | Published : 2017
Interleukin (IL)11 is a crucial factor for human trophoblast function and placentation. Elevated levels are associated with pregnancy complications including preeclampsia, intrauterine growth restriction (IUGR) and preterm birth. However, the regulation of IL11 in the placenta has not been investigated. We examined the effect of pro-inflammatory cytokines IL1β and TNFα, as well as low oxygen tension (2%) on IL11 levels in first trimester placental villous explants. IL1β upregulated IL11 mRNA and protein, while TNFα and low oxygen had no effect. Using mass spectrometry, we identified protein disulfide isomerase 4 (PDIA4) in IL11-treated first trimester human placental explants (100 ng/ml, 24 ..View full abstract
Awarded by NHMRC Australia Project
Awarded by NHMRC Australia Senior Research Fellowship
This work was supported by NHMRC Australia Project Grant (1078674) and the Victorian Government's Operational Infrastructure Support Program. AW was supported by a Cancer Council Victoria Postdoctoral Fellowship. ED was supported by a NHMRC Australia Senior Research Fellowship (550905).