Transcytosis of IL-11 and Apical Redirection of gp130 Is Mediated by IL-11 alpha Receptor
Niloufar Monhasery, Jens Moll, Carly Cuman, Manuel Franke, Larissa Lamertz, Rebecca Nitz, Boris Goerg, Dieter Haussinger, Juliane Lokau, Doreen M Floss, Roland Piekorz, Eva Dimitriadis, Christoph Garbers, Juergen Scheller
CELL REPORTS | CELL PRESS | Published : 2016
Interleukin (IL)-11 signaling is involved in various processes, including epithelial intestinal cell regeneration and embryo implantation. IL-11 signaling is initiated upon binding of IL-11 to IL-11R1 or IL-11R2, two IL-11α-receptor splice variants, and gp130. Here, we show that IL-11 signaling via IL-11R1/2:gp130 complexes occurs on both the apical and basolateral sides of polarized cells, whereas IL-6 signaling via IL-6R:gp130 complexes is restricted to the basolateral side. We show that basolaterally supplied IL-11 is transported and released to the apical extracellular space via transcytosis in an IL-11R1-dependent manner. By contrast, IL-6R and IL-11R2 do not promote transcytosis. In ad..View full abstract
Awarded by Deutsche Forschungsgemeinschaft
Awarded by National Health and Medical Research Council (NHMRC) of Australia
This work was funded by grants from the Deutsche Forschungsgemeinschaft (DFG SCHE907/3-1 and SFB974 A12 to J.S. and GA2048/1-1 and SFB877 project A10 to C.G.), the National Health and Medical Research Council (NHMRC) of Australia Senior Research Fellowship (ID 1019826 to E.D.), and the Victorian Infrastructure Support Program and Australian Government NHMRC IRIISS (to E.D.).