Soluble Delta-like ligand 1 alters human endometrial epithelial cell adhesive capacity
Michelle Van Sinderen, Jennifer Oyanedel, Ellen Menkhorst, Carly Cuman, Katarzyna Rainczuk, Amy Winship, Lois Salamonsen, Tracey Edgell, Evdokia Dimitriadis
REPRODUCTION FERTILITY AND DEVELOPMENT | CSIRO PUBLISHING | Published : 2017
The endometrium undergoes substantial morphological and functional changes to become receptive to embryo implantation and to enable establishment of a successful pregnancy. Reduced Delta-like ligand 1 (DLL1, Notch ligand) in the endometrium is associated with infertility. DLL1 can be cleaved by 'a disintegrin and metalloprotease' (ADAM) proteases to produce a soluble ligand that may act to inhibit Notch signalling. We used an enzyme-linked immunosorbent assay to quantify soluble DLL1 in uterine lavages from fertile and infertile women in the secretory phase of the menstrual cycle. We also determined the cellular location and immunostaining intensity of ADAM12 and 17 in human endometrium thro..View full abstract
Awarded by NHMRC (Australia)
Awarded by NHMRC (Australia) Research Fellowships
This work was supported by a NHMRC (Australia) Project Grant (# 1027100) and by the Victorian Government's Operational Infrastructure Support Program. E.D. and L.A.S. were supported by NHMRC (Australia) Research Fellowships (# 550905 and ## 1102028, respectively).