Journal article
Leukemia inhibitory factor enhances endometrial stromal cell decidualization in humans and mice
LL Shuya, EM Menkhorst, J Yap, P Li, N Lane, E Dimitriadis
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2011
Open access
Abstract
Adequate differentiation or decidualization of endometrial stromal cells (ESC) is critical for successful pregnancy in humans and rodents. Here, we investigated the role of leukemia inhibitory factor (LIF) in human and murine decidualization. Ex vivo human (H) ESC decidualization was induced by estrogen (E, 10 -8 M) plus medroxyprogesterone acetate (MPA, 10 -7 M). Exogenous LIF (≥50 ng/ml) induced STAT3 phosphorylation in non-decidualized and decidualized HESC and enhanced E+MPA-induced decidualization (measured by PRL secretion, P100 pg/mg G-CSF, IL6, IL8, and MCP1. Decidualized HESC secreted IL6, IL8, IL15 and MCP1. LIF (50 ng/ml) up-regulated IL6 and IL15 (P<0.05) secretion in decidualize..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
This work was supported by the Consortium for Industrial Collaboration in Contraceptive Research Program of the Contraceptive Research and Development Program, Eastern Virginia Medical School (Sub-project CIG-07-116), the National Health and Medical Research Council (Australia) fellowships to ED (#550905) and EM (#611827), and the Victorian (Australia) Government's Operational Infrastructure Support Program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.