Journal article

Immunohistochemical measurement of tumor vascular endothelial growth factor in breast cancer - A more reliable predictor of tumor stage than microvessel density or serum vascular endothelial growth factor

G Callagy, E Dimitriadis, J Harmey, D Bouchier-Hayes, M Leader, E Kay

APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY | LIPPINCOTT WILLIAMS & WILKINS | Published : 2000

Abstract

Microvessel density counting is commonly proposed as a method of assessing angiogenesis. However, results have been difficult to reproduce because of many methodological inconsistencies. Vascular endothelial growth factor (VEGF), an angiogenic regulator, is also a poor prognostic indicator in breast cancer, correlating in many studies with microvessel density. In this study, VEGF and microvessel density counting were examined as methods of assessing angiogenesis in breast cancer and correlated with tumor stage. A representative tumor section was stained with anti-CD34 and anti-VEGF. Microvessel density was evaluated using the manual "hot-spot" procedure and a semiquantitative image analysis ..

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