3D organoid-derived human glomeruli for personalised podocyte disease model ling and drug screening
Lorna J Hale, Sara E Howden, Belinda Phipson, Andrew Lonsdale, Pei X Er, Irene Ghobrial, Salman Hosawi, Sean Wilson, Kynan T Lawlor, Shahnaz Khan, Alicia Oshlack, Catherine Quinlan, Rachel Lennon, Melissa H Little
Nature Communications | NATURE PUBLISHING GROUP | Published : 2018
Awarded by Wellcome Trust
Awarded by National Health and Medical Research Council of Australia
Awarded by National Institutes of Health
We thank Moin Saleem, University of Bristol for the provision of the conditionally immortalised podocyte cell line. RNA sequencing was performed by the Translational Genomics Unit, Murdoch Children's Research Institute. We thank the family of the patient described in this study and we acknowledge the KidGen consortium, particularly Andrew Mallett, for recruitment ethics. iPSC patient line derivation was performed by iPSC Core Facility, Murdoch Children's Research Institute. Mass spectrometry was performed in the Biomolecular Analysis Core Facility, University of Manchester, UK and supported by fellowship funding (to R.L.) from the Wellcome Trust (202860/Z/16/Z). M. C.R.I. is supported by the Victorian Government's Operational Infrastructure Support Program. We also thank the Centre for Advanced Histology and Microscopy at the Peter MacCallum Cancer Centre for assistance with scanning electron microscopy. A.O. is an NHMRC Career Development Fellow. M.H.L. is an NHMRC Senior Principal Research Fellow (GNT1136085). This work was supported by the National Health and Medical Research Council of Australia (GNT1098654), the National Institutes of Health (DK107344-01) and the Royal Children's Hospital Foundation for funding of the ReGeniPS program.