Journal article
Whole Genome Sequencing of Australian Candida glabrata Isolates Reveals Genetic Diversity and Novel Sequence Types
C Biswas, VR Marcelino, S Van Hal, C Halliday, E Martinez, Q Wang, S Kidd, K Kennedy, D Marriott, CO Morrissey, I Arthur, K Weeks, MA Slavin, TC Sorrell, V Sintchenko, W Meyer, SCA Chen
Frontiers in Microbiology | FRONTIERS MEDIA SA | Published : 2018
Open access
Abstract
Candida glabrata is a pathogen with reduced susceptibility to azoles and echinocandins. Analysis by traditional multilocus sequence typing (MLST) has recognized an increasing number of sequence types (STs), which vary with geography. Little is known about STs of C. glabrata in Australia. Here, we utilized whole genome sequencing (WGS) to study the genetic diversity of 51 Australian C. glabrata isolates and sought associations between STs over two time periods (2002–2004, 2010–2017), and with susceptibility to fluconazole by principal component analysis (PCA). Antifungal susceptibility was determined using Sensititre YeastOneTM Y010 methodology and WGS performed on the NextSeq 500 platform (I..
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Funding Acknowledgements
SV, MS, DM, CM, KK, TS, SK, CH, and IA are part of antifungal advisory boards and untied grants from MSD Australia and Gilead Sciences Inc.