Multiplex Droplet Digital PCR Assay for Quantification of Human T-Cell Leukemia Virus Type 1 Subtype c DNA Proviral Load and T Cells from Blood and Respiratory Exudates Sampled in a Remote Setting
David Yurick, Georges Khoury, Bridle Clemens, Liyen Loh, Hai Pham, Katherine Kedzierska, Lloyd Einsiedel, Damian Purcell
JOURNAL OF CLINICAL MICROBIOLOGY | AMER SOC MICROBIOLOGY | Published : 2019
During human T-cell leukemia virus type 1 (HTLV-1) infection, the frequency of cells harboring an integrated copy of viral cDNA, the proviral load (PVL), is the main risk factor for progression of HTLV-1-associated diseases. Accurate quantification of provirus by droplet digital PCR (ddPCR) is a powerful diagnostic tool with emerging uses for monitoring viral expression. Current ddPCR techniques quantify HTLV-1 PVL in terms of whole genomic cellular material, while the main targets of HTLV-1 infection are CD4+ and CD8+ T cells. Our understanding of HTLV-1 proliferation and the amount of viral burden present in different compartments is limited. Recently a sensitive ddPCR assay was applied to..View full abstract
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Awarded by National Health and Medical Research Council of Australia (NHMRC) program
Awarded by NHMRC
We sincerely thank all the remote Indigenous Australian community members who participated in this study. We also thank members of the scientific community who generously shared reagents critical to this work. We acknowledge Kim Wilson of the National Reference Laboratory of Melbourne, Australia, and gratefully acknowledge the support of the Pathology Department at Alice Springs Hospital. We also thank the DMI Flow Facility staff for their advice and generous assistance during the sorting experiments.This study was supported by the National Health and Medical Research Council of Australia (NHMRC) program grant number 1052979 to D.P. and program grant number 1071916 to K.K. K.K. is a NHMRC Senior Research Level B Fellow (1102792), and B.C. is a NHMRC Peter Doherty Fellow.