DNA methylation dynamics in the germline of the marsupial tammar wallaby, Macropus eugenii
Teruhito Ishihara, Danielle Hickford, Geoff Shaw, Andrew J Pask, Marilyn B Renfree
DNA RESEARCH | OXFORD UNIV PRESS | Published : 2019
Parent specific-DNA methylation is the genomic imprint that induces mono-allelic gene expression dependent on parental origin. Resetting of DNA methylation in the germ line is mediated by a genome-wide re-methylation following demethylation known as epigenetic reprogramming. Most of our understanding of epigenetic reprogramming in germ cells is based on studies in mice, but little is known about this in marsupials. We examined genome-wide changes in DNA methylation levels by measuring 5-methylcytosine expression, and mRNA expression and protein localization of the key enzyme DNA methyltransferase 3 L (DNMT3L) during germ cell development of the marsupial tammar wallaby, Macropus eugenii. Our..View full abstract
This study was supported by funds from the Norma Hilda Schuster (nee Swift) Scholarship from the University of Melbourne to T.I. and the School of BioSciences, The University of Melbourne.