Cell quiescence correlates with enhanced glioblastoma cell invasion and cytotoxic resistance
Ryan J Atkins, Stanley S Stylli, Natalie Kurganovs, Stefano Mangiola, Cameron J Nowell, Thomas M Ware, Niall M Corcoran, Daniel V Brown, Andrew H Kaye, Andrew Morokoff, Rodney B Luwor, Christopher M Hovens, Theo Mantamadiotis
EXPERIMENTAL CELL RESEARCH | ELSEVIER INC | Published : 2019
Glioblastoma (GBM) tumor cells exhibit drug resistance and are highly infiltrative. GBM stem cells (GSCs), which have low proliferative capacity are thought to be one of the sources of resistant cells which result in relapse/recurrence. However, the molecular mechanisms regulating quiescent-specific tumor cell biology are not well understood. Using human GBM cell lines and patient-derived GBM cells, Oregon Green dye retention was used to identify and isolate the slow-cycling, quiescent-like cell subpopulation from the more proliferative cells in culture. Sensitivity of cell subpopulations to temozolomide and radiation, as well as the migration and invasive potential were measured. Differenti..View full abstract
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Awarded by NHMRC, Australia
The study received funding from The Royal Melbourne Hospital Neuroscience Foundation, Australia and NHMRC, Australia project grants 1104010 (C.M.H., N.M.C.) and 1047581 (C.M.H., N.M.C.), as well as a federal grant from the Australian Department of Health and Aging to the Epworth Cancer Centre, Australia (N.M.C., C.M.H.).