Intersectin-1 interacts with the golgin GCC88 to couple the actin network and Golgi architecture
Christian Makhoul, Prajakta Gosavi, Regina Duffield, Bronwen Delbridge, Nicholas A Williamson, Paul A Gleeson
MOLECULAR BIOLOGY OF THE CELL | AMER SOC CELL BIOLOGY | Published : 2019
The maintenance of the Golgi ribbon relies on a dynamic balance between the actin and microtubule networks; however, the pathways controlling actin networks remain poorly defined. Previously, we showed that the trans-Golgi network (TGN) membrane tether/golgin, GCC88, modulates the Golgi ribbon architecture. Here, we show that dispersal of the Golgi ribbon by GCC88 is dependent on actin and the involvement of nonmuscle myosin IIA. We have identified the long isoform of intersectin-1 (ITSN-1), a guanine nucleotide exchange factor for Cdc42, as a novel Golgi component and an interaction partner of GCC88 responsible for mediating the actin-dependent dispersal of the Golgi ribbon. We show that pe..View full abstract
Awarded by Australian Research Council
Confocal microscopy was performed at the Biological Optical Microscopy Platform (BOMP) at the University of Melbourne. This work was supported by funding from the Australian Research Council (DP100102394). We gratefully acknowledge Fiona Houghton for reagents, technical advice, and suggestions and Lou Fourriere-Chea for expert advice on live imaging.