Mapping Surface Hydrophobicity of α-Synuclein Oligomers at the Nanoscale.
Ji-Eun Lee, Jason C Sang, Margarida Rodrigues, Alexander R Carr, Mathew H Horrocks, Suman De, Marie N Bongiovanni, Patrick Flagmeier, Christopher M Dobson, David J Wales, Steven F Lee, David Klenerman
Nano Letters: a journal dedicated to nanoscience and nanotechnology | Published : 2018
Proteins fold into a single structural ensemble but can also misfold into many diverse structures including small aggregates and fibrils, which differ in their toxicity. The aggregate surface properties play an important role in how they interact with the plasma membrane and cellular organelles, potentially inducing cellular toxicity, however, these properties have not been measured to date due to the lack of suitable methods. Here, we used a spectrally resolved, super-resolution imaging method combined with an environmentally sensitive fluorescent dye to measure the surface hydrophobicity of individual aggregates formed by the protein α-synuclein (αS), whose aggregation is associated with P..View full abstract
Awarded by Biotechnology and Biological Sciences Research Council
Awarded by Medical Research Council