Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria
Rudolf O Schlechter, Hyunwoo Jun, Michal Bernach, Simisola Oso, Erica Boyd, Dian A Munoz-Lintz, Renwick CJ Dobson, Daniela M Remus, Mitja NP Remus-Emsermann
FRONTIERS IN MICROBIOLOGY | FRONTIERS MEDIA SA | Published : 2018
Differential fluorescent labeling of bacteria has become instrumental for many aspects of microbiological research, such as the study of biofilm formation, bacterial individuality, evolution, and bacterial behavior in complex environments. We designed a variety of plasmids, each bearing one of eight unique, constitutively expressed fluorescent protein genes in conjunction with one of four different antibiotic resistance combinations. The fluorophores mTagBFP2, mTurquoise2, sGFP2, mClover3, sYFP2, mOrange2, mScarlet-I, and mCardinal, encoding for blue, cyan, green, green-yellow, yellow, orange, red, and far-red fluorescent proteins, respectively, were combined with selectable markers conferri..View full abstract
Awarded by Royal Society of New Zealand Marsden Fast Start grant
This work was funded by a seed grant of the Biomolecular Interaction Centre of the University of Canterbury to RD and MR-E and the Royal Society of New Zealand Marsden Fast Start grant (UOC1704) to MR-E. RS was supported by a NZIDRS doctoral scholarship. MB was supported by a University of Canterbury Doctoral Scholarship and a Biomolecular Interaction Centre doctoral scholarship.