Journal article

Overexpression of rice aquaporin OsPIP1;2 improves yield by enhancing mesophyll CO2 conductance and phloem sucrose transport

Feiyun Xu, Ke Wang, Wei Yuan, Weifeng Xu, Liu Shuang, Herbert J Kronzucker, Guanglei Chen, Rui Miao, Maoxing Zhang, Ming Ding, Liang Xiao, Lei Kai, Jianhua Zhang, Yiyong Zhu

Journal of Experimental Botany | Oxford University Press (OUP) | Published : 2019

Abstract

Aquaporins are involved in CO2 transport from the leaf intercellular air space to the chloroplast, which contributes to CO2 assimilation. However, the mechanism of CO2 transport by rice (Oryza sativa L.) aquaporins is unknown. Here, we investigated the function of the aquaporin OsPIP1;2 in CO2 diffusion-associated photosynthesis and phloem sucrose transport. Moreover, the grain yield of rice lines overexpressing OsPIP1;2 was determined. OsPIP1;2 was localized to the plasma membrane and the relative expression of OsPIP1;2 was approximately 5-fold higher in leaves in the presence of an elevated CO2 concentration. Overexpression of OsPIP1;2 increased mesophyll conductance by approximately 150% ..

View full abstract

University of Melbourne Researchers

Grants

Awarded by National Key R&D Program of China


Awarded by National Natural Science Foundation of China


Funding Acknowledgements

We are grateful for grant support from the National Key R&D Program of China (2018YFD0200302 and 2018YFD020044), National Natural Science Foundation of China (31471937 and 31761130073). Furthermore, we thank Professor Ian Dodd (Lancaster University) for useful discussion on this work.