Journal article
The inner ear proteome of fish
ORB Thomas, SE Swearer, EA Kapp, P Peng, GQ Tonkin-Hill, A Papenfuss, A Roberts, P Bernard, BR Roberts
FEBS Journal | WILEY | Published : 2019
DOI: 10.1111/febs.14715
Abstract
The mechanisms that underpin the formation, growth and composition of otoliths, the biomineralized stones in the inner ear of fish, are largely unknown, as only a few fish inner ear proteins have been reported. Using a partial transcriptome for the inner ear of black bream (Acanthopagrus butcheri), in conjunction with proteomic data, we discovered hundreds of previously unknown proteins in the otolith. This allowed us to develop hypotheses to explain the mechanisms of inorganic material supply and daily formation of growth bands. We further identified a likely protein mediator of crystal nucleation and an explanation for the apparent metabolic inertness of the otolith. Due to the formation o..
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Awarded by Tunghai University
Funding Acknowledgements
We thank G. Jenkins, M. Sievers, K. Hassell, J. French, D. Chamberlain and L. Barrett for assistance with sample collection and S. Mukherjee for assistance with laboratory work. We thank the Plant Genome & Molecular Evolution Lab, Department of Life Science, Tunghai University, Taiwan for use of their supercomputer, and Steven Wilcox at the Systems Biology Division at the Walter and Eliza Hall Institute of Medical Research for advice and sequencing of cDNA. We similarly thank Karin Limburg for her advice and comments. Funding was provided by the Australian Research Council (project LP140100087) and the University of Melbourne (John and Allan Gilmour Research Award). ORBT was supported by an Australian Postgraduate Award.