Ab initio phasing of the diffraction of crystals with translational disorder
Andrew J Morgan, Kartik Ayyer, Anton Barty, Joe PJ Chen, Tomas Ekeberg, Dominik Oberthuer, Thomas A White, Oleksandr Yefanov, Henry N Chapman
ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES | INT UNION CRYSTALLOGRAPHY | Published : 2019
To date X-ray protein crystallography is the most successful technique available for the determination of high-resolution 3D structures of biological molecules and their complexes. In X-ray protein crystallography the structure of a protein is refined against the set of observed Bragg reflections from a protein crystal. The resolution of the refined protein structure is limited by the highest angle at which Bragg reflections can be observed. In addition, the Bragg reflections alone are typically insufficient (by a factor of two) to determine the structure ab initio, and so prior information is required. Crystals formed from an imperfect packing of the protein molecules may also exhibit conti..View full abstract
Awarded by NSF STC Award
We acknowledge support through the Gottfried Wilhelm Leibniz Program of the DFG. JPJC also acknowledges support from NSF STC Award 1231306. AJM acknowledges the support of the Australian Research Council Centre of Excellence in Advanced Molecular Imaging (AMI).