HIV latency can be established in proliferating and nonproliferating resting CD4( ) T cells in vitro: implications for latency reversal
Michael A Moso, Jenny L Anderson, Samantha Adikari, Lachlan R Gray, Georges Khoury, Judy J Chang, Jonathan C Jacobson, Anne M Ellet, Wan-Jung Chen, Suha Saleh, John J Zaunders, Damian FJ Purcell, Paul U Camerona, Melissa J Churchill, Sharon R Lewin, Hao K Lu
AIDS | LIPPINCOTT WILLIAMS & WILKINS | Published : 2019
OBJECTIVE: To determine whether latency can be established and reversed in both proliferating and nonproliferating CD4+ T cells in the same model in vitro. METHODS: Activated CD4+ T cells were infected with either a nonreplication competent, luciferase reporter virus or wild-type full-length enhanced green fluorescent protein (EGFP) reporter virus and cultured for 12 days. The cells were then sorted by flow cytometry to obtain two distinct T-cell populations that did not express the T-cell activation markers, CD69, CD25 and human leukocyte antigen (HLA)-DR: CD69CD25HLA-DR small cells (nonblasts) that had not proliferated in vitro following mitogen stimulation and CD69CD25HLA-DR large cells (..View full abstract
Awarded by National Institute of Allergy and Infectious Disease (NIAID), US National Institutes of Health (NIH) (Delaney AIDS Research Enterprise, DARE)
The authors declare that they have no competing interests. S.R.L. is an Australian National Health and Medical Research Council (NHMRC) Practitioner Fellow. This work was supported by a program grant from the Australian NHMRC; the National Institute of Allergy and Infectious Disease (NIAID), US National Institutes of Health (NIH) (Delaney AIDS Research Enterprise, DARE; U19AI096109 and RFA-AI-15-029) and the American Foundation for AIDS Research.