Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults
Katerina Vlahos, Koula Sourris, Robyn Mayberry, Penelope McDonald, Freya F Bruveris, Jacqueline V Schiesser, Kiymet Bozaoglu, Paul J Lockhart, Edouard G Stanley, Andrew G Elefanty
STEM CELL RESEARCH | ELSEVIER | Published : 2019
We describe the generation and characterization of 5 human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. The iPSC lines exhibited a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. These iPSC lines can be used as controls in studying disease mechanisms.
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Awarded by Australian National Health & Medical Research Council
The Murdoch Children's Research Institute iPSC Core Facility was funded by a generous donation from the Stafford Fox Medical Research Foundation. This study was funded in part by Australian National Health & Medical Research Council research fellowships awarded to A.G.E. (GNT1117596) and E.G.S (GNT1079004) and a project grant awarded to P.J.L and E.G.S. (GNT1098255). Additional infrastructure funding to the Murdoch Children's Research Institute was provided by the Australian Government National Health and Medical Research Council Independent Research Institute Infrastructure Support Scheme and the Victorian Government's Operational Infrastructure Support Program.