Oligopeptides Generated by Neprilysin Degradation of beta-Amyloid Have the Highest Cu(II) Affinity in the Whole A beta Family
Karolina Bossak-Ahmad, Mariusz Mital, Dawid Plonka, Simon C Drew, Wojciech Bal
INORGANIC CHEMISTRY | AMER CHEMICAL SOC | Published : 2019
The catabolism of β-amyloid (Aβ) is carried out by numerous endopeptidases including neprilysin, which hydrolyzes peptide bonds preceding positions 4, 10, and 12 to yield Aβ4-9 and a minor Aβ12- x species. Alternative processing of the amyloid precursor protein by β-secretase also generates the Aβ11- x species. All these peptides contain a Xxx-Yyy-His sequence, also known as an ATCUN or NTS motif, making them strong chelators of Cu(II) ions. We synthesized the corresponding peptides, Phe-Arg-His-Asp-Ser-Gly-OH (Aβ4-9), Glu-Val-His-His-Gln-Lys-am (Aβ11-16), Val-His-His-Gln-Lys-am (Aβ12-16), and pGlu-Val-His-His-Gln-Lys-am (pAβ11-16), and investigated their Cu(II) binding properties using pote..View full abstract
Awarded by National Science Centre of Poland
The study was sponsored by the National Science Centre of Poland, Grant No. 2014/15/B/ST5/05229 (W.B.). The equipment used was sponsored in part by the Centre for Preclinical Research and Technology (CePT), a project cosponsored by the European Regional Development Fund and Innovative Economy, The National Cohesion Strategy of Poland. S.C.D. was supported by a fellowship awarded by the faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne.