Hyaluronan coated cerium oxide nanoparticles modulate CD44 and reactive oxygen species expression in human fibroblasts
Megan S Lord, Brooke L Farrugia, Claudia MY Yan, James A Vassie, John M Whitelock
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | WILEY | Published : 2016
Cerium oxide nanoparticles are being widely explored for cell therapies. In this study, nanoceria was functionalized with hyaluronan (HA) using the organosilane linker, 3-aminopropyltriethoxysilane. HA-nanoceria was found to be cytocompatible and to reduce intracellular reactive oxygen species in human fibroblasts. The HA-nanoceria was found to colocalize with CD44 on the surface of the cells and once internalized traffic to the lysosomes, be degraded and induce markers of autophagy. These particles were also effective in reducing the cell surface expression of CD44. Together these data suggest that HA-nanoceria is a promising drug delivery material to target CD44-expressing cells through a ..View full abstract
Awarded by Australian Research Council
Contract grant sponsor: Australian Research Council under the Discovery Project; contract grant number: DP1097149.