Journal article

The localisation of inflammatory cells and expression of associated proteoglycans in response to implanted chitosan

Brooke L Farrugia, John M Whitelock, MoonSun Jung, Barbara McGrath, Robert L O'Grady, Simon J McCarthy, Megan S Lord



Implantation of a foreign material almost certainly results in the formation of a fibrous capsule around the implant however, mechanistic events leading to its formation are largely unexplored. Mast cells are an inflammatory cell type known to play a role in the response to material implants, through the release of pro-inflammatory proteases and cytokines from their α-granules following activation. This study examined the in vivo and in vitro response of mast cells to chitosan, through detection of markers known to be produced by mast cells or involved with the inflammatory response. Mast cells, identified as Leder stained positive cells, were shown to be present in response to material impl..

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University of Melbourne Researchers


Awarded by Australian Research Council

Funding Acknowledgements

This work was supported by funding from the Australian Research Council under the Linkage Project (LP0776293) scheme. The authors acknowledge Prof. Bruce Caterson, Cardiff University, UK, for the supply of the CS stub antibody (clone 2B6) and Prof. Achilleas Theocharis, University of Patras, Greece, for the supply of the polyclonal rabbit anti-serglycin antibody. The authors also acknowledge Dr Christine Chung Heart Research Institute, for assistance in histological staining.