Synthesis and characterization of water soluble biomimetic chitosans for bone and cartilage tissue regeneration
Megan S Lord, Bonny M Tsoi, Brooke L Farrugia, SR Simon Ting, Shenda Baker, William P Wiesmann, John M Whitelock
JOURNAL OF MATERIALS CHEMISTRY B | ROYAL SOC CHEMISTRY | Published : 2014
Chitosan, a polysaccharide derived from the exoskeleton of crustaceans, insects, the cell walls of fungi, the radulas of mollusks and the internal shells of cephalopods, has been shown to promote osteogenesis. Arginine functionalized chitosan, a water soluble derivative of chitosan, was successfully sulfated with a degree of sulfur incorporation of up to 9% with substitution at the 2-N position. This degree of sulfation replicated those of naturally occurring growth factor binding glycosaminoglycans. Sulfated chitosan-arginine was found to bind and signal fibroblast growth factor 2. Chitosan-arginine promoted an osteogenic phenotype in primary human fetal chondroblasts over a period of 7 day..View full abstract
Awarded by Australian Research Council
This work was supported by funding from the Australian Research Council under the Linkage Project (LP100100504) scheme. The authors would also like to acknowledge the UNSW Mark Wainwright Analytical Centre for assistance with NMR analysis.