Chitosan-Based Heparan Sulfate Mimetics Promote Epidermal Formation in a Human Organotypic Skin Model
Brooke L Farrugia, Yaolei Mi, Ha Na Kim, John M Whitelock, Shenda M Baker, William P Wiesmann, Zhe Li, Peter Maitz, Megan S Lord
Advanced Functional Materials | WILEY-V C H VERLAG GMBH | Published : 2018
Biomimetic materials that replicate biological functions have great promise for use as therapeutics in regenerative medicine applications. Heparan sulfate (HS) is the natural binding partner for growth factors enabling longer half-lives and potentiation of their signaling. In this study a water soluble chitosan-arginine is modified with sulfate moieties in order to mimic the structure of HS. Sulfated chitosan-arginine with a degree of sulfation of 58% binds fibroblast growth factor 2 with higher affinity than HS. The sulfated chitosan-arginine also promotes epithelial cell migration and supports the formation of an expanded epidermis in an organotypic skin model. Furthermore, sulfated chitos..View full abstract
Awarded by Australian Research Council
This work was supported by funding from the Australian Research Council under the Linkage Project (LP100100504) scheme. The authors also acknowledge the UNSW Mark Wainwright Analytical Centre for assistance with NMR and XPS analyses. Ch-Arg is a proprietary molecule covered by patents US 8,119,780, US 8,658,775, AU 2007257436, and AU 20133211460.