Does osteogenic potential of clonal human bone marrow mesenchymal stem/stromal cells correlate with their vascular supportive ability?
Alison T Merryweather-Clarke, David Cook, Barbara Joo Lara, Peng Hua, Emmanouela Repapi, Neil Ashley, Shiang Y Lim, Suzanne M Watt
STEM CELL RESEARCH & THERAPY | BMC | Published : 2018
BACKGROUND: Human bone marrow-derived mesenchymal stem/stromal cells (hBM MSCs) have multiple functions, critical for skeletal formation and function. Their functional heterogeneity, however, represents a major challenge for their isolation and in developing potency and release assays to predict their functionality prior to transplantation. Additionally, potency, biomarker profiles and defining mechanisms of action in a particular clinical setting are increasing requirements of Regulatory Agencies for release of hBM MSCs as Advanced Therapy Medicinal Products for cellular therapies. Since the healing of bone fractures depends on the coupling of new blood vessel formation with osteogenesis, w..View full abstract
Awarded by Wellcome Trust
Awarded by National Institute of Health Research
Awarded by EU Merlin FP7 grant
We thank the High-Throughput Genomics Group at the Wellcome Trust Centre for Human Genetics (funded by Wellcome Trust grant reference 090532/Z/09/Z) for the generation of the sequencing data. SMW received grant support for these studies from the National Institute of Health Research under its Programme Grants Scheme (RP-PG-0310-1001 and -1003), the NHSBT Trust Fund, NHS Blood and Transplant, Restore Burns and Wound Healing Trust, EU Merlin FP7 grant (602363) and the University of Oxford. PH received support for these studies from the University of Oxford. The views expressed in this publication are those of the authors and not necessarily those of NHS, NHS Blood and Transplant, NIHR or the Department of Health.