Journal article

Staphylococcus aureus Superantigen-Like Protein SSL1: A Toxic Protease

Aihua Tang, Armando R Caballero, Michael A Bierdeman, Mary E Marquart, Timothy J Foster, Ian R Monk, Richard J O'Callaghan

PATHOGENS | MDPI | Published : 2019

Abstract

Staphylococcus aureus is a major cause of corneal infections that can cause reduced vision, even blindness. Secreted toxins cause tissue damage and inflammation resulting in scars that lead to vision loss. Identifying tissue damaging proteins is a prerequisite to limiting these harmful reactions. The present study characterized a previously unrecognized S. aureus toxin. This secreted toxin was purified from strain Newman ΔhlaΔhlg, the N-terminal sequence determined, the gene cloned, and the purified recombinant protein was tested in the rabbit cornea. The virulence of a toxin deletion mutant was compared to its parent and the mutant after gene restoration (rescue strain). The toxin (23 kDa) ..

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University of Melbourne Researchers

Grants

Awarded by National Eye Institute of the National Institutes of Health


Awarded by Science Foundation Ireland


Funding Acknowledgements

This research was funded in part by the National Eye Institute of the National Institutes of Health (EY010974) and the Science Foundation Ireland (08/IN.1/B1845).