Journal article

Adaptations of a Competitive Enzyme-Linked Immunosorbent Assays for the Detection of Antibodies to Influenza A Virus in Horse Sera for Use in Wild Aquatic Birds

MA Hoque, LF Skerratt, S Garland, GW Burgess, P Selleck

INDIAN JOURNAL OF VIROLOGY | SPRINGER INDIA | Published : 2012

Abstract

We applied a competitive enzyme-linked immunosorbent assay for the detection of antibodies for influenza A in equine sera to their detection in sera from wild aquatic birds. Suboptimal results were obtained for the optical density (OD) of the monoclonal antibody (MAb) control and reproducibility between duplicate analyses in the initial assessment. It was therefore necessary to modify the assay to deliver increased reliability and reproducibility while maintaining adequate sensitivity. We optimized reagent concentrations to obtain optimal OD values (close to 2) for the monoclonal antibody control and used 2, 2'-Azino-bis: 3-Benzthiazoline-6-Sulphonic Acid as an alternative chromogen to poten..

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University of Melbourne Researchers

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Funding Acknowledgements

We are very grateful to the Australian Biosecurity Cooperative Research Centre (ABCRC) and Australian Department of Agriculture, Forestry and Fisheries (DAFF) for providing project financial support. We thank AusAID for providing an Australian Leadership Award (ALA) and the ABCRC for a top-up PhD scholarship to MAH. We extend our thanks to the scientific and ethics permit authorities for providing the following project approvals, Eco-access permit No. WISp04374507, Queensland Parks and Wildlife Service, northern Region and ethics permit No. A 1175, JCU. Finally, we extend our heartfelt thanks to the members of our AI research team and other contributors to our research for their excellent co-operation during the study period.