Journal article
Polymorphic repetitive loci of the amphibian pathogen Batrachochytrium dendrobatidis
S Garland, TY James, D Blair, L Berger, LF Skerratt
Diseases of Aquatic Organisms | INTER-RESEARCH | Published : 2011
DOI: 10.3354/dao02396
Abstract
Batrachochytrium dendrobatidis (Bd), the cause of a fatal fungal skin disease ofamphibians that has led to massive die-offs, global declines and extinctions, has spread internationallyas a pandemic clone with low genetic diversity. A need exists to develop highly polymorphicmarkers to determine centers of origin and patterns of spread to assist in the development ofmanagement strategies. Comparison of paralogous sequences, obtained from the 2 sequenced Bdgenomes, indicates useful levels of inter-strain polymorphism in repetitive fragments. Weassessed 6 repetitive loci for variation within and among Australian isolates using standard fragmentanalysis and capillary electrophoresis-single stran..
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Funding Acknowledgements
Thanks to the anonymous reviewers for constructive comments and M. D. Kusrini, Bogor Agricultural University, Bogor, Indonesia, for supply of the Indonesian sample. This study was made possible due to funding from the Australian Government Department of the Environment, Water, Heritage and the Arts and James Cook University.