Monitoring of wild birds for Newcastle disease virus in north Queensland, Australia
MA Hoque, GW Burgess, D Karo-Karo, AL Cheam, LF Skerratt
PREVENTIVE VETERINARY MEDICINE | ELSEVIER SCIENCE BV | Published : 2012
Wild aquatic birds (WABs) are considered as reservoir hosts for Newcastle disease viruses (NDVs) and may act as vectors for transferring these viruses to poultry, causing outbreaks of disease. A 3-year epidemiological study was conducted on WABs of north Queensland from April 2007 to March 2010. Swab and fresh moist faecal samples of WABs were screened to detect Newcastle disease viral (NDV) RNA by one-step real time reverse transcriptase polymerase chain reaction (rRT-PCR) in multiplex primers, targeting the matrix gene. The potential reactor samples in rRT-PCR were processed for sequencing of the different NDV genes using conventional PCR. The overall NDV RNA prevalence was 3.5% for live b..View full abstract
We are very grateful to the Australian Biosecurity Cooperative Research Centre (AB-CRC) and the Australian Department of Agriculture, Forestry and Fisheries for providing project financial support. We thank AusAID for providing an Australian Leadership Award and the AB-CRC for a top-up PhD scholarship to MAH. We extend our thanks to scientific and ethics permit authorities for providing the following project approvals, Eco-access permit no. WISp04374507, Queensland Parks and Wildlife Service, Northern Region and ethics permit no. A1175, JCU. Finally, we extend our heartfelt thanks to the members of our AI research team and other contributors to our research for their excellent co-operation during the study period.