Epigenetic and Transcriptome Profiling Identifies a Population of Visceral Adipose-Derived Progenitor Cells with the Potential to Differentiate into an Endocrine Pancreatic Lineage
Michael D Williams, Mugdha Joglekar, Sarang N Satoor, Wilson Wong, Effie Keramidaris, Amanda Rixon, Philip O'Connell, Wayne J Hawthorne, Geraldine M Mitchell, Anandwardhan A Hardikar
Cell Transplantation | SAGE PUBLICATIONS INC | Published : 2019
Type 1 diabetes (T1D) is characterized by the loss of insulin-producing β-cells in the pancreas. T1D can be treated using cadaveric islet transplantation, but this therapy is severely limited by a lack of pancreas donors. To develop an alternative cell source for transplantation therapy, we carried out the epigenetic characterization in nine different adult mouse tissues and identified visceral adipose-derived progenitors as a candidate cell population. Chromatin conformation, assessed using chromatin immunoprecipitation (ChIP) sequencing and validated by ChIP-polymerase chain reaction (PCR) at key endocrine pancreatic gene promoters, revealed similarities between visceral fat and endocrine ..View full abstract
The support provided to MDW and WW through the Australian Government's post-graduate awards, MVJ through JDRF International post-doctoral fellowship, SNS through an NHMRC post-doctoral grant, EK and AR through the O'Brien Institute, GM through the NHMRC and O'Brien Institute and AAH through the ARC Future Fellowship and currently through the JDRF Australia Career Development Award is highly acknowledged. The authors acknowledge the surgical team at SVHM, Melbourne for providing the adipose tissues and Prof. Edouard G. Stanley and Prof. Andrew Elefanty (CMRI, Melbourne) for the generous gift of Pdx1-GFP reporter mice. The infrastructure support provided to AAH through the NHMRC Clinical Trials Center, The University of Sydney, The Rebecca Cooper Medical Research Foundation and the O'Brien Institute/St Vincent's Hospital, Melbourne is highly acknowledged.