Proteomic analysis of the molecular response of Raji cells to maslinic acid treatment
WH Yap, KS Khoo, SH Lim, CC Yeo, YM Lim
PHYTOMEDICINE | ELSEVIER GMBH | Published : 2012
Maslinic acid, a natural pentacyclic triterpene has been shown to inhibit growth and induce apoptosis in some tumour cell lines. We studied the molecular response of Raji cells towards maslinic acid treatment. A proteomics approach was employed to identify the target proteins. Seventeen differentially expressed proteins including those involved in DNA replication, microtubule filament assembly, nucleo-cytoplasmic trafficking, cell signaling, energy metabolism and cytoskeletal organization were identified by MALDI TOF-TOF MS. The down-regulation of stathmin, Ran GTPase activating protein-1 (RanBP1), and microtubule associated protein RP/EB family member 1 (EB1) were confirmed by Western blott..View full abstract
Awarded by MOSTI
The authors would like to express their deepest gratitude to Universiti Tunku Abdul Rahman and MOSTI e-science fund (00-02-11-SF0028) for supporting this project.