Investigating Neural Stem Cell and Glioma Stem Cell Self-renewal Potential Using Extreme Limiting Dilution Analysis (ELDA)
T Mantamadiotis, Hong Nguyen, Paul Daniel, gulay Filiz
BIO-PROTOCOL | Bio-Protocol | Published : 2018
Glioma stem cells (GSC) grown as neurospheres exhibit similar characteristics to neural stem cells (NSC) grown as neurospheres, including the ability to self-renew and differentiate. GSCs are thought to play a role in cancer initiation and progression. Self-renewal potential of GSCs is thought to reflect many characteristics associated with malignancy, including tumor recurrence following cytotoxic therapy due to their proliferative dormancy and capacity to allow for the development of resistant tumor cell sub-clones due to mutations acquired during their differentiation. Here, we demonstrate that using extreme limiting dilution analysis (ELDA), subtle differences in the frequency of sphere-..View full abstract
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Awarded by CASS Foundation
This work was supported by the CASS Foundation Grant (#7491). A brief description of the protocol described in this article originally appeared in Daniel et al. (2017 and 2018). The original ELDA protocol is described in Hu and Smyth, 2009.