Journal article

Long-term organic fertilization increased antibiotic resistome in phyllosphere of maize

Qing-Lin Chen, Xin-Li An, Bang-Xiao Zheng, Yi-Bing Ma, Jian-Qiang Su

SCIENCE OF THE TOTAL ENVIRONMENT | ELSEVIER SCIENCE BV | Published : 2018

Abstract

Phyllosphere contains various microorganisms that may harbor diverse antibiotic resistance genes (ARGs). However, we know little about the composition of antibiotic resistome and the factors influencing the diversity and abundance of ARGs in the phyllosphere. In this study, 16S rRNA gene amplicon sequencing and high-throughput quantitative PCR approaches were employed to investigate the effects of long-term (over 10 years) organic fertilization on the phyllosphere bacterial communities and antibiotic resistome. Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes dominated in the phyllosphere bacterial communities. Long-term application of sewage sludge and chicken manure altered the..

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University of Melbourne Researchers

Grants

Awarded by National Key Research and Development Plan of China


Awarded by Natural Science Foundation of China


Funding Acknowledgements

This work was supported by the National Key Research and Development Plan of China (2016YFD0800205, 2017YFE0107300), the Natural Science Foundation of China (31722004), the K.C. Wong Education Foundation and the Youth Innovation Promotion Association, CAS.