Long-term organic fertilization increased antibiotic resistome in phyllosphere of maize
Qing-Lin Chen, Xin-Li An, Bang-Xiao Zheng, Yi-Bing Ma, Jian-Qiang Su
SCIENCE OF THE TOTAL ENVIRONMENT | ELSEVIER SCIENCE BV | Published : 2018
Phyllosphere contains various microorganisms that may harbor diverse antibiotic resistance genes (ARGs). However, we know little about the composition of antibiotic resistome and the factors influencing the diversity and abundance of ARGs in the phyllosphere. In this study, 16S rRNA gene amplicon sequencing and high-throughput quantitative PCR approaches were employed to investigate the effects of long-term (over 10 years) organic fertilization on the phyllosphere bacterial communities and antibiotic resistome. Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes dominated in the phyllosphere bacterial communities. Long-term application of sewage sludge and chicken manure altered the..View full abstract
Awarded by National Key Research and Development Plan of China
Awarded by Natural Science Foundation of China
This work was supported by the National Key Research and Development Plan of China (2016YFD0800205, 2017YFE0107300), the Natural Science Foundation of China (31722004), the K.C. Wong Education Foundation and the Youth Innovation Promotion Association, CAS.