Distinct effects of struvite and biochar amendment on the class 1 integron antibiotic resistance gene cassettes in phyllosphere and rhizosphere
Xin-Li An, Qing-Lin Chen, Dong Zhu, Jian-Qiang Su
SCIENCE OF THE TOTAL ENVIRONMENT | ELSEVIER | Published : 2018
Struvite recovered from wastewater is promising for recycling phosphorus into soil as fertilizers. However, struvite application may prompt the proliferation of antibiotic resistance in soil and plant. This study examined the impacts of struvite application and biochar amendment on integrons abundance and gene cassette contexts in rhizosphere soil and phyllosphere using quantitative PCR and clone library analysis. Microcosm experiments revealed that class 1 integron was the most prevalent in all samples, with higher concentration and higher relative abundance in rhizosphere than those in phyllosphere. The majority of resistance gene cassettes were associated with genes encoding resistance to..View full abstract
Awarded by Natural Science Foundation of China
Awarded by National Key Research and Development Program of China-International collaborative project from Ministry of Science and Technology
Awarded by Knowledge Innovation Program of the Chinese Academy of Sciences
This study was financially supported by the Natural Science Foundation of China (31722004), the National Key Research and Development Program of China-International collaborative project from Ministry of Science and Technology (2017YFE0107300), the Knowledge Innovation Program of the Chinese Academy of Sciences (IUEQN201504), K. C. Wong Education Foundation and Youth Innovation Promotion Association, CAS.