Do manure-borne or indigenous soil microorganisms influence the spread of antibiotic resistance genes in manured soil?
Qing-Lin Chen, Xin-Li An, Hu Li, Yong-Guan Zhu, Jian-Qiang Su, Li Cui
SOIL BIOLOGY & BIOCHEMISTRY | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2017
Manure application is a common practice that not only adds nutrients and organic matter to arable soils for crop growth, but also introduces antibiotic resistance genes (ARGs), posing a potential risk to human health. To investigate the mechanisms underlying the spread of ARGs in manured soil, especially the impact of manure-borne and indigenous soil microorganisms, a microcosm experiment with four specially designed treatments over a period of two months was conducted, including soil, soil with irradiated pig manure, irradiated soil with pig manure, and soil with pig manure. A total of 240 unique ARGs were detected via a high-throughput quantitative PCR (HT-qPCR) targeting almost all major ..View full abstract
Awarded by National Natural Science Foundation of China
Awarded by Knowledge Innovation Program of the Chinese Academy of Sciences
Awarded by Youth Innovation Promotion Association, CAS
This work was supported by the National Natural Science Foundation of China (21210008, 41571130063), the Knowledge Innovation Program of the Chinese Academy of Sciences IUEQN20150 and the Youth Innovation Promotion Association, CAS (2014266).