Species determination of Malaysian Bactrocera pests using PCR-RFLP analyses (Diptera: Tephritidae)
Tock H Chua, Yi Vern Chong, Saw Hoon Lim
PEST MANAGEMENT SCIENCE | JOHN WILEY & SONS LTD | Published : 2010
BACKGROUND: Identification of Bactrocera carambolae Drew and Hancock, B. papayae Drew and Hancock, B. tau Walker, B. latifrons Hendel, B. cucurbitae Coquillett, B. umbrosa Fabricius and B. caudata Fabricius would pose a problem if only a body part or an immature stage were available. Analysis of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of cytochrome oxidase I (COI) gene using primers COIR, COIF, UEA7 and UEA10 and restriction enzymes (MseI, RsaI and Alu1) was carried out. The banding profiles in the electrophoresis gel were analysed. RESULTS: The COI gene in six Bactrocera spp. was successfully amplified by COIR and COIF, as well as UEA7 and UEA10, while ..View full abstract
Awarded by Ministry of Science and Technology
The authors thank the Ministry of Science and Technology for funding the research (E Science Fund: 211046-116-00) and Monash University for providing general facilities for conducting the research. They would like to thank their colleague Dr Song Beng Kah for helping in the analysis. They are also grateful to the anonymous reviewer, who gave several constructive suggestions.