Unique Transcriptional Architecture in Airway Epithelial Cells and Macrophages Shapes Distinct Responses following Influenza Virus Infection Ex Vivo
Joel Z Ma, Wy Ching Ng, Luke Zappia, Linden J Gearing, Moshe Olshansky, Kym Pham, Karey Cheong, Arthur Hsu, Stephen J Turner, Odilia Wijburg, Sarah L Londrigan, Andrew G Brooks, Patrick C Reading
JOURNAL OF VIROLOGY | AMER SOC MICROBIOLOGY | Published : 2019
Airway epithelial cells and macrophages differ markedly in their responses to influenza A virus (IAV) infection. To investigate transcriptional responses underlying these differences, purified subsets of type II airway epithelial cells (ATII) and alveolar macrophages (AM) recovered from the lungs of mock- or IAV-infected mice at 9 h postinfection were subjected to RNA sequencing. This time point was chosen to allow for characterization of cell types first infected with the virus inoculum, prior to multicycle virus replication and the infiltration of inflammatory cells into the airways. In the absence of infection, AM predominantly expressed genes related to immunity, whereas ATII expressed g..View full abstract
Related Projects (2)
Awarded by National Health and Medical Research Council (NHMRC) of Australia
This study was supported by project grant APP1044976 and program grant APP1071916 from the National Health and Medical Research Council (NHMRC) of Australia. The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health.