Journal article

The Microtubule-Associated Innate Immune Sensor GEF-H1 Does Not Influence Mouse Norovirus Replication in Murine Macrophages

Svenja Fritzlar, Peter A White, Jason M Mackenzie

VIRUSES-BASEL | MDPI | Published : 2019

Abstract

Norovirus is an acute infection of the gastrointestinal tract causing rapid induction of vomiting and diarrhoea. The infection is sensed and controlled by the innate immune system, particularly by the RNA helicase MDA-5 and type I and III interferons (IFNs). We have observed that intracellular replication of murine norovirus (MNV) occurs in membranous clusters proximal to the microtubule organising centre, a localisation dependent on intact microtubules. Recently, it was shown that the host protein guanine nucleotide exchange factor-H1 (GEF-H1) is a microtubule-associated innate immune sensor that activates interferon Regulatory Factor 3 to induce the production of type I IFNs. Thus, we inte..

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Grants

Awarded by National Health and Medical Research Council of Australia


Funding Acknowledgements

We thank Turgut Aktepe and Lorena Brown (Department of Microbiology and Immunology, University of Melbourne) for providing the West Nile virus and IAV x31 virus, respectively. We thank Hans-Christian Reinecker (Harvard Medical School, Boston) for generously providing the GEF-H1 cDNA expression plasmids. We would like to thank Kim Green for generously providing anti-MNV antibodies. We would like to thank the Biological Optical Microscopy Platform (BOMP) at the University of Melbourne for their assistance with this project. This study has been supported by project grant Nos. 1083139 and 1123135 awarded to J.M.M. and P.A.W. from the National Health and Medical Research Council of Australia.