An ex vivo human placental vessel perfusion method to study mesenchymal stem/stromal cell migration.
Balta Al-Sowayan, Rosemary J Keogh, Mohammed Abumaree, Harry M Georgiou, Bill Kalionis
Stem Cell Investig | Published : 2019
Background: To initiate tissue repair, mesenchymal stem/stromal cells (MSCs) must enter the blood stream, migrate to the targeted area, cross the endothelial barrier and home to the damaged tissue. This process is not yet fully understood in humans and thus, the aim of this study was to develop an ex vivo placental vessel perfusion method to examine human MSC movement from a blood vessel into human tissue. This will provide a better understanding of MSC migration, movement through the endothelial barrier and engraftment into target tissue, in a setting that more closely represents the in vivo state, compared with conventional in vitro human cell culture models. Moreover, important similariti..View full abstract