Characterization of insulin and IGF-1 receptor binding in equine liver and lamellar tissue: implications for endocrinopathic laminitis
SN Nanayakkara, S Rahnama, PA Harris, ST Anderson, MA de Laat, S Bailey, MN Sillence
Domestic Animal Endocrinology | Elsevier | Published : 2019
Although it is well established that equine laminitis can be triggered by extreme hyperinsulinemia, the mechanism of insulin action is not known. High concentrations of insulin lead to separation of the weight-bearing apparatus from the hoof wall and are associated with an increased cycle of cell death and proliferation in the lamellae. Gene expression and immunohistochemistry studies have indicated that the lamellae are sparsely populated with insulin receptors, whereas IGF-1 receptors (IGF-1R) are abundant, suggesting that the action of insulin may be mediated by insulin binding to the IGF-1R. To investigate this possibility, cell membrane fragments containing IGF-1R were extracted from th..View full abstract
This study was funded by MARS Petcare, Queensland University of Technology and the Australian Research Council. All the authors had a role in the study design, including P.A Harris who is an employee of MARS Petcare. S.N.N. and S.R. executed the experiment. All authors contributed to data interpretation and article preparation. All authors approved the final version of this article. The authors declare no conflicts of interest.