IN VIVO CHARACTERIZATION OF COLORECTAL AND CUTANEOUS INPUTS TO LUMBOSACRAL DORSAL HORN NEURONS IN THE MOUSE SPINAL CORD
KE Farrell, MM Rank, S Keely, AM Brichta, BA Graham, RJ Callister
NEUROSCIENCE | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2016
Chronic abdominal pain is a common symptom of inflammatory bowel disease and often persists in the absence of gut inflammation. Although the mechanisms responsible for ongoing pain are unknown, clinical and preclinical evidence suggests lumbosacral spinal cord dorsal horn neurons contribute to these symptoms. At present, we know little about the intrinsic and synaptic properties of this population of neurons in either normal or inflammed conditions. Therefore, we developed an in vivo preparation to make patch-clamp recordings from superficial dorsal horn (SDH) neurons receiving colonic inputs in naïve male mice. Recordings were made in the lumbosacral spinal cord (L6-S1) under isoflurane ane..View full abstract
Awarded by National Health and Medical Research Council of Australia
This work was supported by grants from the National Health and Medical Research Council of Australia (APP1067146 to R.J.C.), the Hunter Medical Research Institute and the University of Newcastle Priority Research Centre for Translational Neuroscience.