Journal article

Effect of immunosuppressive drugs on cytokine production in canine whole blood stimulated with lipopolysaccharide or a combination of ionomycin and phorbol 12-myristate 13-acetate

Julien RS Dandrieux, Lakshmi Narayanan, Simon Firestone, Todd M Archer, Caroline S Mansfield

Veterinary Medicine and Science | John Wiley & Sons | Published : 2019

Abstract

A pharmacodynamic assay has been previously developed to monitor ciclosporin treatment in dogs by assessing inhibition of cytokine transcription after whole blood stimulation with 12-myristate 13-1 acetate and ionomycin (PMA/I). In this study, whole blood stimulation with either PMA/I or lipopolysaccharide (LPS) was used to assess the effect of multiple drugs (azathioprine, ciclosporin, mycophenolate, leflunomide and prednisone) after a 7-day treatment course on production of cytokines measured with a multiplex assay in healthy dogs (n = 4 for each treatment). Interleukin-10 (IL-10), interferon gamma (IFN?) and tumour necrosis factor alpha (TNFa) were significantly activated by PMA/I stimula..

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Grants

Awarded by Australian Research Council (ARC) Discovery Early Career Research Award


Awarded by NIH COBRE grant


Funding Acknowledgements

JRSD travel costs were partially funded by the Sunshine Foundation Scholarship from the University of Melbourne. SF is supported by an Australian Research Council (ARC) Discovery Early Career Research Award (DE160100477). The FCCF is partially supported by NIH COBRE grant 5P20GM103646-04.