Development and Application of Droplet Digital PCR Tools for the Detection of Transgenes in Pastures and Pasture-Based Products
Paula A Giraldo, Noel O Cogan, German C Spangenberg, Kevin F Smith, Hiroshi Shinozuka
FRONTIERS IN PLANT SCIENCE | FRONTIERS MEDIA SA | Published : 2019
Implementation of molecular biotechnology, such as transgenic technologies, in forage species can improve agricultural profitability through achievement of higher productivity, better use of resources such as soil nutrients, water, or light, and reduced environmental impact. Development of detection and quantification techniques for genetically modified plants are necessary to comply with traceability and labeling requirements prior to regulatory approval for release. Real-time PCR has been the standard method used for detection and quantification of genetically modified events, and droplet digital PCR is a recent alternative technology that offers a higher accuracy. Evaluation of both techn..View full abstract
This work was supported by funding from the Victorian Department of Economic Development, Jobs, Transport and Resources, Dairy Australia and an Australian Government Research Training Program Scholarship for PG.