Divergence of regulatory networks governed by the orthologous transcription factors FLC and PEP1 in Brassicaceae species
Julieta L Mateos, Vicky Tilmes, Pedro Madrigal, Edouard Severing, Rene Richter, Colin WM Rijkenberg, Pawel Krajewski, George Coupland
Proceedings of the National Academy of Sciences | NATL ACAD SCIENCES | Published : 2017
Genome-wide landscapes of transcription factor (TF) binding sites (BSs) diverge during evolution, conferring species-specific transcriptional patterns. The rate of divergence varies in different metazoan lineages but has not been widely studied in plants. We identified the BSs and assessed the effects on transcription of FLOWERING LOCUS C (FLC) and PERPETUAL FLOWERING 1 (PEP1), two orthologous MADS-box TFs that repress flowering and confer vernalization requirement in the Brassicaceae species Arabidopsis thaliana and Arabis alpina, respectively. We found that only 14% of their BSs were conserved in both species and that these contained a CArG-box that is recognized by MADS-box TFs. The CArG-..View full abstract
Awarded by Deutsche Forschungsgemeinschaft via the Cluster of Excellence on Plant Science
Awarded by EU Marie Curie Innovative Training Network
We thank Uciel Chorostecki from the Institute of Molecular and Cell Biology of Rosario, Argentina, for bioinformatics assistance and Wen-Biao Jiao and Korbinian Schneeberger for providing the Arabis montbretiana genome assembly and annotation. This study was supported by the Alexander von Humboldt-Stiftung and Argentinean National Council of Sciences (J.L.M.), Deutsche Forschungsgemeinschaft via the Cluster of Excellence on Plant Science Grant EXC 1028 (to V.T.), EU Marie Curie Innovative Training Network SYSFLO 237909 (to P.M. and P.K.), the ERC through HyLife (G.C.), and a core grant of the Max Planck Society (to G.C.). Some computations were performed at the Poznan Supercomputing and Networking Center.