Journal article
Single-cell analysis reveals congruence between kidney organoids and human fetal kidney
AN Combes, L Zappia, PX Er, A Oshlack, MH Little
Genome Medicine | BMC | Published : 2019
Abstract
Background: Human kidney organoids hold promise for studying development, disease modelling and drug screening. However, the utility of stem cell-derived kidney tissues will depend on how faithfully these replicate normal fetal development at the level of cellular identity and complexity. Methods: Here, we present an integrated analysis of single cell datasets from human kidney organoids and human fetal kidney to assess similarities and differences between the component cell types. Results: Clusters in the combined dataset contained cells from both organoid and fetal kidney with transcriptional congruence for key stromal, endothelial and nephron cell type-specific markers. Organoid enriched ..
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Grants
Awarded by Murdoch Children's Research Institute
Funding Acknowledgements
This work was supported by the Australian Research Council (DE150100652), the National Health and Medical Research Council (NHMRC) of Australia (GNT1156567), National Institutes of Health Rebuilding a Kidney consortium (DK107344) and seed funding from the Murdoch Children's Research Institute and the University of Melbourne. A.N.C. was supported by a Discovery Early Career Researcher Award from the Australian Research Council. M.H.L. is a Senior Principal Research Fellow of the NHMRC (GNT1136085). A.O. is a Career Development Fellow of the NHMRC. L.Z. is supported by an Australian Government Research Training Program (RTP) Scholarship. MCRI is supported by the Victorian Government's Operational Infrastructure Support Program. These funding bodies had no role in any aspect of the study design, execution, or writing.