Determination of haemoglobin derivatives in aged dried blood spot to estimate haematocrit
Rosita Zakaria, Katrina J Allen, Jennifer J Koplin, Nick Crinis, Lidia De Rosa, Peter Roche, Ronda F Greaves
CLINICAL CHEMISTRY AND LABORATORY MEDICINE | WALTER DE GRUYTER GMBH | Published : 2019
Introduction Dried blood spot (DBS) sample applications now encompass analytes related to clinical diagnosis, epidemiological studies, therapeutic drug monitoring, pharmacokinetic and toxicokinetic studies. Haematocrit (Hct) and haemoglobin (Hb) at very high or low concentrations may influence the accuracy of measurement quantification of the DBS sample. In this study, we aimed to predict the Hct of the punched DBS through primary spectrophotometric estimation of its haemoglobin-derivative (Hb-drv) content. Methods Formic acid solution was used to elute Hb-drv content of 3.2 mm spotted blood from its dry matrix. Direct spectrometry measurement was utilised to scan the extracted Hb-drv in the..View full abstract
Awarded by NHMRC - Centre of Excellence in Paediatric Food Allergy and Food-related Immune Disorders
NHMRC - Centre of Excellence in Paediatric Food Allergy and Food-related Immune Disorders - Funder Id: 10.13039/501100000925, Grant ID 1041420.