Journal article

Method to Synchronize Cell Cycle of Human Pluripotent Stem Cells without Affecting Their Fundamental Characteristics.

Loukia Yiangou, Rodrigo A Grandy, Carola M Morell, Rute A Tomaz, Anna Osnato, Juned Kadiwala, Daniele Muraro, Jose Garcia-Bernardo, Shota Nakanoh, William G Bernard, Daniel Ortmann, Davis J McCarthy, Ingrid Simonic, Sanjay Sinha, Ludovic Vallier

Stem Cell Reports | Published : 2019

Abstract

Cell cycle progression and cell fate decisions are closely linked in human pluripotent stem cells (hPSCs). However, the study of these interplays at the molecular level remains challenging due to the lack of efficient methods allowing cell cycle synchronization of large quantities of cells. Here, we screened inhibitors of cell cycle progression and identified nocodazole as the most efficient small molecule to synchronize hPSCs in the G2/M phase. Following nocodazole treatment, hPSCs remain pluripotent, retain a normal karyotype and can successfully differentiate into the three germ layers and functional cell types. Moreover, genome-wide transcriptomic analyses on single cells synchronized fo..

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University of Melbourne Researchers

Grants

Awarded by British Heart Foundation


Awarded by National Centre for the Replacement, Refinement and Reduction of Animals in Research


Awarded by Medical Research Council


Awarded by Wellcome Trust