Journal article

The Arabidopsis AMOT1/EIN3 gene plays an important role in the amelioration of ammonium toxicity

Guangjie Li, Lin Zhang, Meng Wang, Dongwei Di, Herbert J Kronzucker, Weiming Shi

JOURNAL OF EXPERIMENTAL BOTANY | OXFORD UNIV PRESS | Published : 2019

Abstract

Ammonium (NH4+) toxicity inhibits shoot growth in Arabidopsis, but the underlying mechanisms remain poorly characterized. Here, we show that a novel Arabidopsis mutant, ammonium tolerance 1 (amot1), exhibits enhanced shoot growth tolerance to NH4+. Molecular cloning revealed that amot1 is a new allele of EIN3, a key regulator of ethylene responses. The amot1 mutant and the allelic ein3-1 mutants show greater NH4+ tolerance than the wild type. Moreover, transgenic plants overexpressing EIN3 (EIN3ox) are more sensitive to NH4+ toxicity The ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) increases shoot sensitivity to NH4+, whereas the ethylene perception inhibitor Ag+ decreases ..

View full abstract

University of Melbourne Researchers

Grants

Awarded by National Natural Science Foundation of China


Awarded by National Key R&D Program of China


Awarded by Chinese Academy of Sciences Innovation Program


Funding Acknowledgements

We thank Dr J. Alonso at North Carolina State University for providing seeds of the EBS: GUS reporter lines which were generated by Dr Anna Stepanova in Dr Joe Ecker's lab. We thank Dr Hongwei Guo for providing the seeds of ein3-1, eil1-1, ein3eil1, and EIN3ox transgenic lines, and the ABRC of Ohio State University for etr1-3, eto1-1, and rbohd mutant seeds. This work was supported by the National Natural Science Foundation of China (no. 31430095), the National Key R&D Program of China (no. 2017YFD0200103), the Chinese Academy of Sciences Innovation Program (no. CAS ISSASIP1604), and the University of Melbourne.