Conference Proceedings

Acoustic streaming driven enhanced dye-uptake in cells for fluorescence imaging

Melanie EM Stamp, Wei Tong, David J Garrett, Christoph Westerhausen, Steven Prawer, Achim Wixforth

2018 IEEE INTERNATIONAL ULTRASONICS SYMPOSIUM (IUS) | IEEE | Published : 2018

Abstract

The major control function in all types of neurons is based on versatile intracellular signals generated by calcium ions. These ions control heart functions as well as the regulation of vital aspects of the entire cell cycle. Calcium imaging is a powerful tool for observing the activity of neurons in neural tissue and for monitoring the effectiveness of electrical stimulation devices. In regard to neuronal prostheses, calcium imagine is used to quantify the electric stimulated interaction between implant and ganglions. Although different techniques exist for staining retinas, most of them lead to cell death or a too low concentration. In this study, we propose to use guided ultrasound to gen..

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Funding Acknowledgements

This work was performed in part at the Melbourne Centre for Nanofabrication (MCN) in the Victorian Node of the Australian National Fabrication Facility (ANFF). This research was supported by the National Health and Medical Research Council (NHMRC) and the Australian Research Council (ARC) through its Special Research Initiative (SRI) in Bionic Vision Science and Technology grant to the Bionic Vision Australia (BVA). Furthermore, the authors thank the "Deutsche Forschungs Gemeinschaft (DFG)" and Nanosystems Initiative Munich (NIM) for the financial support.