Journal article

CCL27/CCL28-CCR10 Chemokine Signaling Mediates Migration of Lymphatic Endothelial Cells

Tara Karnezis, Rae H Farnsworth, Nicole C Harris, Steven P Williams, Carol Caesar, David J Byrne, Prad Herle, Maria L Macheda, Ramin Shayan, You-Fang Zhang, Sezer Yazar, Simon J Takouridis, Craig Gerard, Stephen B Fox, Marc G Achen, Steven A Stacker



Metastasis via the lymphatic vasculature is an important step in cancer progression. The formation of new lymphatic vessels (lymphangiogenesis), or remodeling of existing lymphatics, is thought to facilitate the entry and transport of tumor cells into lymphatic vessels and on to distant organs. The migration of lymphatic endothelial cells (LEC) toward guidance cues is critical for lymphangiogenesis. While chemokines are known to provide directional navigation for migrating immune cells, their role in mediating LEC migration during tumor-associated lymphangiogenesis is not well defined. Here, we undertook gene profiling studies to identify chemokine-chemokine receptor pairs that are involved ..

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Funding Acknowledgements

The authors thank Sally Roufail for expert technical assistance and Andrew Naughton, Jacinta Carter and Animal Facility staff at the Ludwig Institute for Cancer Research (Melbourne) and Peter MacCallum Cancer Centre for assistance with mouse experiments; Janna Taylor for assistance in generating figures; Jason Li for bioinformatics assistance; and Bao Lu of the Boston Children's Hospital, Harvard Medical School, for providing Ccr10<SUP>-/-</SUP> mouse tissues. The authors also acknowledge the support and resources of the Centre for Advanced Histology and Microscopy at the Peter MacCallum Cancer Centre (A/Prof. Sarah Ellis, Marne Prinsloo, Thu Ming Noc Nguyen, Ethan Passantino, Metta Jana, Jill Danne, Cameron Skinner, Dhanya Menon), along with imaging assistance from Stephen Cody, Cameron Nowell, and Naomi Campanale. This work was funded partly by a Program Grant from the National Health and Medical Research Council of Australia (NHMRC). S.A. Stacker and M.G. Achen are supported by NHMRC Senior Research Fellowships. S.A. Stacker would like to acknowledge the support of the Pfizer Australia Fellowship. R. Shayan is supported by the Raelene Boyle Sporting Chance Foundation and Royal Australasian College of Surgeons (RACS) Foundation Scholarship, and the RACS Surgeon Scientist Program.